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anti cd4  (Bio X Cell)


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    Structured Review

    Bio X Cell anti cd4
    Anti Cd4, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 97/100, based on 777 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+cd4/InVivoMAb+anti-mouse+CD4/pm42864360-447-3-11
    Average 97 stars, based on 777 article reviews
    anti cd4 - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Infection:

    Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection
    Article Snippet: .. Depletion of CD4+ cells in chronically infected μMT -/- mice was performed by injecting a single dose of 300 μg of anti-mouse CD4, clone GK1.5 (BioXCell #BE0003–1) intraperitoneally. .. The isotype control group received the same dose of rat IgG2b isotype anti-keyhole limpet hemocyanin, clone LTF-2 (BioXCell #BE0090) as previously described [ ].

    Article Title: Interleukin-10 limits immune-mediated pathology in chronic subclinical plasmodial infection.
    Article Snippet: .. Depletion of CD4+ cells in chronically infected μMT-/- mice was performed by injecting a single dose of 300 μg of anti-mouse CD4, clone GK1.5 (BioXCell #BE0003–1) intraperitoneally. .. The isotype control group received the same dose of rat IgG2b isotype anti- keyhole limpet hemocyanin, clone LTF-2 (BioXCell #BE0090) as previously described [21].

    Mouse Assay:

    Article Title: Suppression of RBC alloimmunization and regulation of CD4 + T cell dependence by C3 is not due to genetic confounders in mice.
    Article Snippet: Funding information National Institutes of Health, Grant/Award Number: P01HL169552 Abstract Background: Activation of complement protein C3 generally enhances antibody responses and C3-null mice have decreased antibody-based immunity.. Mener et al. have reported a paradoxical suppressor function for C3 in alloimmunization to transfused RBCs as alloantibodies are increased in C3-null mice.. Moreover, C3 regulated the CD4 T cell dependence of the immune response.

    Control:

    Article Title: Suppression of RBC alloimmunization and regulation of CD4 + T cell dependence by C3 is not due to genetic confounders in mice.
    Article Snippet: Funding information National Institutes of Health, Grant/Award Number: P01HL169552 Abstract Background: Activation of complement protein C3 generally enhances antibody responses and C3-null mice have decreased antibody-based immunity.. Mener et al. have reported a paradoxical suppressor function for C3 in alloimmunization to transfused RBCs as alloantibodies are increased in C3-null mice.. Moreover, C3 regulated the CD4 T cell dependence of the immune response.

    Article Title: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity
    Article Snippet: .. For T cell depletion experiments, mice were injected intraperitoneally with 200 μg of anti-mouse CD4 (clone GK1.5, BioXCell BE0003-1) and/or CD8 (clone 2.43, BioXCell BE0061) monoclonal antibodies or of isotype-matched control antibody (clone LTF-2, BioXCell, BE0090) on the indicated days. ..

    Article Title: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity.
    Article Snippet: .. For T cell depletion experiments, mice were injected intraperitoneally with 200 μg of anti-mouse CD4 (clone GK1.5, BioXCell BE0003-1) and/ or CD8 (clone 2.43, BioXCell BE0061) monoclonal antibodies or of isotype-matched control antibody (clone LTF-2, BioXCell, BE0090) on the indicated days. ..

    Injection:

    Article Title: Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell‐Induced Ferroptosis in Ovarian Cancer
    Article Snippet: The intensity of specific protein bands was detected using the Immobilon Western Chemiluminescent HRP Substrate and visualized with the ChemiDoc Imaging System (Bio‐Rad). .. CD4+ and CD8+ T cell depletions were performed by using anti‐mouse CD4 (GK1.5, #BE0003, BioxCell) or CD8α (2.43, #BE0061, BioxCell) specific mAbs, intravenously injected (200 μg per mouse) on days –2 and 0 of tumor inoculation. .. The antibody targeting β‐actin (8H10D10, 33700) and ATF4 (D4B8, #11815) was purchased from the Cell Signaling Technology (Danvers, MA), human METTL5 (#16791‐1‐AP) was purchased from Proteintech Group (Rosemont, IL), mouse METTL5 (#A9217) was purchased from ABclonal (Woburn, MA) and the monoclonal ANTI‐FLAG antibody (M2, #F3165) was purchased from MilliporeSigma.

    Article Title: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity
    Article Snippet: .. For T cell depletion experiments, mice were injected intraperitoneally with 200 μg of anti-mouse CD4 (clone GK1.5, BioXCell BE0003-1) and/or CD8 (clone 2.43, BioXCell BE0061) monoclonal antibodies or of isotype-matched control antibody (clone LTF-2, BioXCell, BE0090) on the indicated days. ..

    Article Title: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity.
    Article Snippet: .. For T cell depletion experiments, mice were injected intraperitoneally with 200 μg of anti-mouse CD4 (clone GK1.5, BioXCell BE0003-1) and/ or CD8 (clone 2.43, BioXCell BE0061) monoclonal antibodies or of isotype-matched control antibody (clone LTF-2, BioXCell, BE0090) on the indicated days. ..

    Recombinase Polymerase Amplification:

    Article Title: Superior In Vivo Efficacy for T Cell Engineering via Citronellol-Tailored mRNA-tLNPs.
    Article Snippet: Efficient in vivo generation of CAR T cells via mRNA-LNP delivery necessitates high-efficiency T cell engineering, yet current systems remain suboptimal.. While lymphoid tropism is essential, it is demonstrated that high tropism alone is insufficient for effective in vivo engineering—some highly tropic LNPs frequently exhibit suboptimal T cell transfection.. Crucially, intrinsic T cell transfection potency emerges as an orthogonal and equally critical determinant, consistent with the need for both T cell specificity and transfection efficiency.

    Purification:

    Article Title: Superior In Vivo Efficacy for T Cell Engineering via Citronellol-Tailored mRNA-tLNPs.
    Article Snippet: Efficient in vivo generation of CAR T cells via mRNA-LNP delivery necessitates high-efficiency T cell engineering, yet current systems remain suboptimal.. While lymphoid tropism is essential, it is demonstrated that high tropism alone is insufficient for effective in vivo engineering—some highly tropic LNPs frequently exhibit suboptimal T cell transfection.. Crucially, intrinsic T cell transfection potency emerges as an orthogonal and equally critical determinant, consistent with the need for both T cell specificity and transfection efficiency.

    Bioprocessing:

    Article Title: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity
    Article Snippet: .. For T cell depletion experiments, mice were injected intraperitoneally with 200 μg of anti-mouse CD4 (clone GK1.5, BioXCell BE0003-1) and/or CD8 (clone 2.43, BioXCell BE0061) monoclonal antibodies or of isotype-matched control antibody (clone LTF-2, BioXCell, BE0090) on the indicated days. ..

    Article Title: Simultaneous STING and lymphotoxin-β receptor activation induces B cell responses in tertiary lymphoid structures to potentiate antitumor immunity.
    Article Snippet: .. For T cell depletion experiments, mice were injected intraperitoneally with 200 μg of anti-mouse CD4 (clone GK1.5, BioXCell BE0003-1) and/ or CD8 (clone 2.43, BioXCell BE0061) monoclonal antibodies or of isotype-matched control antibody (clone LTF-2, BioXCell, BE0090) on the indicated days. ..

    other:

    Article Title: Tumor Intrinsic METTL5 Modulates ATF4 Translation to Prevent T Cell-Induced Ferroptosis in Ovarian Cancer.
    Article Snippet: Spleen tissues from experimental mice were mechanically disrupted into single-cell suspensions, and Ammonium-Chloride-Potassium (ACK) lysis (#A1049201, ThermoFisher Scientific) was performed to remove red blood cells.



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    Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized <t>lymphocytes,</t> with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.
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    Image Search Results


    Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

    Journal: Veterinary Medicine and Science

    Article Title: Rare Feline Gingival Extramedullary Plasmacytoma: Clinical Application of Electrochemotherapy for Local Control

    doi: 10.1002/vms3.71294

    Figure Lengend Snippet: Cytologic, histopathologic, immunohistochemical and flow cytometric findings of the gingival mass and regional lymph nodes. (A) Fine‐needle aspiration cytology of the gingival mass showing a low‐cellularity smear composed of a homogeneous population of round cells. The cells exhibit round to oval, occasionally indented and pleomorphic nuclei, with mild to moderate anisocytosis and anisokaryosis. Some cells contain prominent nucleoli, and occasional multinucleated cells are observed. A single neutrophil is present in the upper right field. (B) Histopathologic examination of the gingival mass demonstrating a densely cellular, poorly demarcated, infiltrative round cell neoplasm with frequent mitotic figures. (C and D) Immunohistochemical staining showing strong and diffuse cytoplasmic immunoreactivity of neoplastic cells for MUM‐1, consistent with extramedullary plasmacytoma. (E) Cytologic evaluation of the submandibular lymph node revealed a predominant population of large, atypical round to ovoid cells with marked nuclear atypia, prominent and multiple nucleoli, binucleation and multinucleation, admixed with small to intermediate‐sized lymphocytes, with one abnormal mitotic figure observed in the field, supporting metastatic involvement of the regional lymph node. (F) Flow cytometric immunophenotyping of the lymph node demonstrating heterogeneous expression of CD5 and CD21, with a predominant population of CD5‐ and CD21‐double‐negative cells, supporting the metastatic involvement of extramedullary plasmacytoma in the regional lymph node. Scale bars: A, E = 10 µm; B, D = 50 µm; C = 200 µm.

    Article Snippet: For flow cytometric analysis, antibodies against CD21 (B‐cell marker; clone CA2.1D6, RPE‐conjugated, Bio‐Rad Antibodies, MCA1781R), CD5 (pan–T‐cell marker; clone FE1.1B11, FITC‐conjugated, Bio‐Rad Antibodies, MCA2038F), CD4 (helper T‐cell marker; clone VPG34, FITC‐conjugated; Bio‐Rad Antibodies, MCA1346F) and CD8 (cytotoxic T‐cell marker; clone VPG9, RPE‐conjugated; Bio‐Rad Antibodies, MCA1347R) were used.

    Techniques: Immunohistochemical staining, Staining, Expressing